Mechanism governing separation in microfluidic pinched flow fractionation devices

We present a computational investigation of the mechanism governing size-based particle separation in microfluidic pinched flow fractionation. We study the behavior of particles moving through a pinching gap (i.e., a constriction in the aperture of a channel) in the Stokes regime (negligible fluid and particle inertia) as a function of particle size. The constriction aperture is created by a plane wall and spherical obstacle, and emulates the pinching segment in pinched flow fractionation devices. The simulation results show that the distance of closest approach between the particle and obstacle surfaces (along a trajectory) decreases with increasing particle size. We then use the distance of closest approach to investigate the effect of short-range repulsive non-hydrodynamic interactions (e.g., solid-solid contact due to surface roughness, electrostatic, or steric repulsion, etc.). We define a critical trajectory as the one in which the minimum particle–obstacle separation is equal to the range of the non-hydrodynamic interactions. The results further show that the initial offset of the critical trajectory (defined as the critical offset) increases with particle size. We interpret the variation of the critical offset with particle size as the basis for size-based microfluidic separation in pinched flow fractionation. We also compare the effect of different driving fields on the particle trajectories; we simulate a constant force driving the particles in a quiescent fluid as well as a freely suspended particles in a pressure-driven flow. We observe that the particles approach closer to the obstacle when driven by a constant force, than those freely suspended in a pressure driven flow (for the same initial offset). On the other hand, the increment in the critical offset (as a function of particle size) is larger in the pressure-driven case than in the force-driven case. Thus, pressure-driven particle separation using pinched flow fractionation would prove more effective than its forcedriven counterpart (e.g., particles settling under gravity through a pinching gap).